eNpHR3.0 or hM4D (From
BrainVTA) viruses were used to manipulate the activity of the projection in the AcbC, RE3g-Cre (From
BrainVTA) /flex-taCasp3-TEVp system was used to induce Cre-dependent and caspase 3 (Casp3)-dependent apoptosis and to selectively ablate engram cells in the target brain nucleus.
The viruses used in this article from BrainVTA are in the table below
|
Calcium Sensors |
AAV9-hsyn:DIO-GCaMP6s-eGFP |
|
CRE Recombinase |
PT-0244 AAV9-TRE3g-Cre |
|
Activity-dependent |
PT-0515 AAV9-TRE3g-ChR2-mCherry
AAV9-TRE3g-histone-mCherry
PT-0312 AAV9-TRE-tight-hM4D-mCherry
PT-0138 AAV9-TRE-tight-hM3D-mCherry |
|
Control |
AAV9-hsyn:DIO-histone-eGFP |
|
Tracing Helper |
PT-0021 AAV9-EF1α:DIO-his-eGFP−2a-TVA
PT-0023 AAV9-EF1α:DIO-RVG |
|
RV |
R01002 RV-ENVA-△G-dsRed |
Pub Date: 2019-11-12,
DOI: 10.1038/s41593-019-0524-y Email: [email protected]
Yiming Zhou, Huiwen Zhu, Zhiyuan Liu, Xi Chen, XiuJuan Su, Chaonan Ma, Zhen Tian, Bing Huang, Enhui Yan, Xing Liu and Lan Ma
The importance of neuronal ensembles, termed engram cells, in storing and retrieving memory is increasingly being appreciated, but less is known about how these engram cells operate within neural circuits. Here we tagged engram cells in the ventral CA1 region of the hippocampus (vCA1) and the core of the nucleus accumbens (AcbC) during cocaine conditioned place preference (CPP) training and show that the vCA1 engram projects preferentially to the AcbC and that the engram circuit from the vCA1 to the AcbC mediates memory recall. Direct activation of the AcbC engram while suppressing the vCA1 engram is sufficient for cocaine CPP. The AcbC engram primarily consists of D1 medium spiny neurons, but not D2 medium spiny neurons. The preferential synaptic strengthening of the vCA1→AcbC engram circuit evoked by cocaine conditioning mediates the retrieval of cocaine CPP memory. Our data suggest that the vCA1 engram stores specific contextual information, while the AcbC D1 engram and its downstream network store both cocaine reward and associated contextual information, providing a potential mechanism by which cocaine CPP memory is stored.
Fig. 1 Cocaine CPP training preferentially increases the synaptic connectivity from the vCA1 engram to AcbC D1 engram cells.
In this study, the researchers utilized cocaine CPP, a model of associative memory of drug reward and related context, to demonstrate that the cocaine engram cells in the vCA1 and the AcbC are required for the storage and retrieval of cocaine CPP memory. The data suggested that the vCA1 engram preferentially projects to the AcbC and encodes distinct contextual information, and that the AcbC engram stores cocaine reward and associated context information. Cocaine CPP conditioning evokes vCA1-engram-input-specific postsynaptic remodeling of the AcbC D1 engram that underlies the storage of cocaine CPP memory.
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