BrainVTA

Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Stable Cell Line Generation
Stable cell lines are used in a variety of research areas such as gene function ( proteins function),  recombinant protein and antibody production, designing diseases models, drug discovery and many other areas. BrainVTA could offer high quality stable cell line generation service including  gene expression stable cell line, gene suppression stable cell line (knock-down or knock-out) or genome editing stable cell line base on eukaryotic expression vector or an integrated lentiviral vector. 
 
 Key informations for stable cell line generation
● Largest collection of expression-ready ORF clones and lentiviral particles reduces turnaround time and ensures high quality.
● A wide variety of cell backgrounds ranging from HEK293 to iPSCs.
● An expert in CRISPR technology.
● A full service provider for stable cell line generation projects.
 
Stable Cell Line Service Details

Gene expression stable cell line generation
● Construct vector
Construct your gene of interest into a suitable expression vector (an eukaryotic expression vector or an integrated lentiviral vector).
● Transient expression
 Perform transfection or transduction on target cells.
• Stable cell clones selection
Resistance to antibiotics or flow cytometry screen stable cell clones.
• Gene expression evaluation
Evaluate the gene expression in stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR, or reporter assays etc.

 
                                                                                           Sample drawing
Gene Knockdown stable cell line generation
• ShRNA construction
 Construct shRNA targeting gene of interest into a suitable expression vector (an eukaryotic expression vector or an integrated lentiviral vector).
● Screening of shRNA efficacy
● Transient expression
 Perform transfection or transduction on target cells.
• Stable cell clones selection
Resistance to antibiotics or flow cytometry screen stable cell clones.
• Validation
Validation RNAi gene silencing by real-time qRT-PCR and/or Western blot.
 
Gene Knock-in stable cell line generation
● gRNA/Donor construction
● Validation of cas9- gRNA efficacy
● Perform transfection or transduction on target cells
● Screening of positive cell clones
● Quality control analysis of stable cell line
 
Gene Knockout stable cell line generation
● gRNA construction
● Validation of cas9- gRNA efficacy
● Perform transfection or transduction on target cells
● Screening of positive cell clones
● Quality control analysis of stable cell line
 
BrainVTA has extensive experience in generation of stable cell lines, and could offer optimum experimental conditions to efficiently produce stable cell lines according to your requirements. If you have any need, just email us at sales@brainvta.com or click the Send Request button.