AAV -GCaMP6m was used to monitor the activity of neurons. AAV –mGFP was used for control.
The viruses used in this article are in the table below
|
Control |
AAV -DIO-mGFP |
|
Calcium sensors |
AAV -DIO-GCaMP6m |
Pub Date: 2019-05-06,
DOI: 10.1007/s12264-019-00379-4，
Email: [email protected]
Yi Li • Zhixiang Liu • Qingchun Guo • Minmin Luo
Fiber photometry is a sensitive and easy way to detect changes in fluorescent signals. The combination of fiber photometry with various fluorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of fiber photometry in biomedical fields, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of fiber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.
Fig. 1 The fiber photometry system.
In this article, the authors describe detailed procedures of fiber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.
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