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Targeting aurora kinase B alleviates spinal microgliosis and
shRNA-AURKB was used for genetic knockdown of the rat AURKB gene,shRNA-empty vector was used as a NC. (All viruses were packaged by BrainVTA)
The viruses used in this article from BrainVTA are in the table below
Custom-Made AAVS  rAAV-U6-shRNA(AURKB)-CMV-EGFP-WPRE-pA
 rAAV-U6-shRNA(empty vector)-CMV-EGFP-WPRE-pA
Yu Shen, Zhuofeng Ding, Shengyun Ma, Yu Zou , Xin Yang, Zijin Ding, Yu Zhang, Xiaoyan Zhu, Michael K E Schäfer, Qulian Guo, Changsheng Huang
Pub Date: 2019-08-26,  DOI: 10.1111/jnc.14883,  Email: [email protected]
Peripheral nerve injury elicits spinal microgliosis, contributing to neuropathic pain. The aurora kinases A (AURKA), B (AURKB), and C (AURKC) are potential therapeutic targets in proliferating cells. However, their role has not been clarified in microglia. The aim of this study was to examine the regulation of aurora kinases and their roles and druggability in spinal microgliosis and neuropathic pain. Sprague–Dawley rats received chronic constriction injury (CCI). Gene expression of aurora kinases A-C was evaluated by quantitative RT-PCR and western blot, respectively, in spinal cords at 1, 3, 7, and 14 days after CCI. AURKB gene and protein expression was up-regulated concomitantly with the development of spinal microgliosis and neuropathic pain. Using lentiviral over-expression and adeno-associated viral knockdown approaches, the function of AURKB was further investigated by western blot, immunohistochemistry, RNA sequencing, and pain behavior tests. We found that AURKB over- expression in naive rats caused spinal microgliosis and pain hypersensitivity, whereas AURKB knockdown reduced microgliosis and alleviated CCI-induced neuropathic pain. Accordingly, RNA sequencing data revealed down-regulation of genes critically involved in signaling pathways associated with spinal microgliosis and neuropathic pain after AURKB knockdown in CCI rats. To examine its therapeutic potential for treatment of neuropathic pain, animals were treated intrathecally with the pharmacological AURKB inhibitor AZD1152-HQPA resulting in the alleviation of CCI-induced pain. Taken together, our findings indicated that AURKB plays a critical role in spinal microgliosis and neuropathic pain. Targeting AURKB may be an efficient method for treatment of neuropathic pain subsequent to peripheral nerve injury.

Fig1. AURKB knockdown prevents CCI-induced spinal microgliosis.
To examine the regulation of aurora kinases and their roles and druggability in spinal microgliosis and neuropathic pain, using a combination of genetic knockdown (From BrainVTA) and over-expression studies, as well as pharmacological inhibition of AURKB in the spinal cord, the authors provide first evidence that AURKB is a critical player and novel therapeutic target in chronic spinal microgliosis and neuropathic pain following peripheral nerve injury.
 
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