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Differential coupling between subcortical calcium and BOLD si
Gcamp6f virus (From BrainVTA) was used for fiber photometry.
The viruses used in this article from BrainVTA are in the table below
Calcium sensors  PT-0147 rAAV-hSyn-Gcamp6f-WPRE-pA
Pub Date: 2019-10-15, DOI: 10.1016/j.neuroimage.2019.07.006 Email: [email protected]
Chuanjun Tong, Jian-kun Dai, Yuyan Chen, Kaiwei Zhang, Yanqiu Feng, Zhifeng Liang
Task based and resting state fMRI has been widely utilized to study brain functions. As the foundation of fMRI, the underlying neural basis of the BOLD signal has been extensively studied, but the detailed mechanism remains elusive, particularly during the resting state. To examine the neurovascular coupling, it is important to simultaneously record neural and vascular signals. Here we developed a novel setup of camera based, scalable simultaneous calcium fiber photometry and fMRI in rats. Using this setup, we recorded calcium signals of superior colliculus (SC) and lateral geniculate nucleus (LGN) and fMRI simultaneously during visual stimulation and the resting state. Our results revealed robust, region-specific coupling between calcium and BOLD signals in the task state and weaker, whole brain correlation in the resting state. Interestingly, the spatial specificity of such correlation in the resting state was improved upon regression of white matter, ventricle signals and global signals in fMRI data. Overall, our results suggest differential coupling of calcium and BOLD signals for subcortical regions between evoked and resting states, and the coupling relationship in the resting state was related with resting state BOLD preprocessing strategies.

Fig. 1 Stimulus evoked and resting state GCaMP based fiber photometry recording.
To examine the neurovascular coupling, the authors developed a novel setup of camera based, scalable simultaneous calcium fiber photometry and fMRI in rats. The results showed robust and region-specific coupling in both SC and LGN for stimulus-related calcium and BOLD signals, suggesting differential coupling of calcium and BOLD signals for subcortical regions between evoked and resting states.
 
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