RV viruses were used for retrograde monosynaptic tracing to label the monosynaptic input neurons of GABAergic neurons in the mPFC. For electrophysiology experiments, hCHR2(H134R) virus was used to assess the functional connectivity among cholinergic neurons, serotoninergic neurons and GABAergic neurons in the mPFC. (all viruses were packaged by
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Tracing Helper |
PT-0095 AAV2/9-EF1a-DIO-histone-BFP-2A-TVA
PT-0062 AAV2/9-EF1a-DIO-GFP-TVA |
|
RV |
R01001 SAD-ΔG-GFP(EnvA)-RV
R01002 SAD-ΔG-DsRed(EnvA)-RV |
|
Optogenetic |
PT-0001 AAV2/9-EF1a-DIO-hCHR2(H134R)-YFP |
Pub Date: 2019-07-08
, DOI: 10.1038/s41593-019-0429-9,
Email: [email protected]
Qingtao Sun, Xiangning Li, Miao Ren, Mengting Zhao, Qiuyuan Zhong, Yuqi Ren, Pan Luo, Hong Ni, Xiaoyu Zhang, Chen Zhang, Jing Yuan, Anan Li, Minmin Luo, Hui Gong and Qingming Luo
The medial prefrontal cortex (mPFC) contains populations of GABAergic interneurons that play different roles in cognition and emotion. Their local and long-range inputs are incompletely understood. We used monosynaptic rabies viral tracers in combination with fluorescence micro-optical sectioning tomography to generate a whole-brain atlas of direct long-range inputs to GABAergic interneurons in the mPFC of male mice. We discovered that three subtypes of GABAergic interneurons in two areas of the mPFC are innervated by same upstream areas. Input from subcortical upstream areas includes cholinergic neurons from the basal forebrain and serotonergic neurons (which co-release glutamate) from the raphe nuclei. Reconstruction of single-neuron morphology revealed novel substantia innominata–anteromedial thalamic nucleus–mPFC and striatum–anteromedial thalamic nucleus–mPFC circuits. Based on the projection logic of individual neurons, we classified cortical and hippocampal input neurons into several types. This atlas provides the anatomical foundation for understanding the functional organization of the mPFC.
Fig.1 monosynaptic rabies viral tracers to investigate the long-range inputs to GABAergic interneurons in mPFC
To investigate the monosynaptic input neurons of GABAergic neurons in the mPFC, the researchers employed monosynaptic rabies viral tracers (From
BrainVTA) in combination with whole-brain precise imaging to generate a whole-brain atlas of direct long-range inputs to GABAergic interneurons in the mPFC. The results showed that the upstream brain areas that project to different subtypes of interneurons in both subregions of the mPFC are similar but with quantitative differences. Furthermore, the researchers identified different types of cortical and hippocampal neurons and novel neural circuits among the mPFC and the upstream input brain areas.
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